Table II.
Recognition and binding of laminarins by rhDectin-1
LaminarinMolecular Mass (Da)Kd (μg/ml)BMAXNonspecifica
01 4840 0.418 (0.254–0.687)b 0.271 (0.191–0.352) 0.00170 (0.00126–0.00214) 
02 4400 0.998c (0.718–1.386) 0.357 (0.283–0.430) none 
02 dialyzed 4400 0.293d (0.182–0.473) 0.223 (0.203–0.248) 0.00235 (0.00214–0.00257) 
03 4800 0.205 (0.110–0.299) 0.0925c (0.0615–0.124) 0.00328 (0.00308–0.00349) 
03 dialyzed 4800 0.446 (0.291–0.683) 0.229d (0.216–0.241) 0.00228 (0.00184–0.00272) 
04 34400 5.618c (3.756–7.475) 0.202 (0.161–0.243) 0.000350 (0.000301–0.000398) 
04 dialyzed 34400 7.820 (5.16–10.48) 0.190 (0.134–0.246) 0.000492 (0.000463–0.000522) 
05 4750 0.402 (0.312–0.491) 0.240 (0.191–0.289) 0.000758 (0.000659–0.000857) 
LaminarinMolecular Mass (Da)Kd (μg/ml)BMAXNonspecifica
01 4840 0.418 (0.254–0.687)b 0.271 (0.191–0.352) 0.00170 (0.00126–0.00214) 
02 4400 0.998c (0.718–1.386) 0.357 (0.283–0.430) none 
02 dialyzed 4400 0.293d (0.182–0.473) 0.223 (0.203–0.248) 0.00235 (0.00214–0.00257) 
03 4800 0.205 (0.110–0.299) 0.0925c (0.0615–0.124) 0.00328 (0.00308–0.00349) 
03 dialyzed 4800 0.446 (0.291–0.683) 0.229d (0.216–0.241) 0.00228 (0.00184–0.00272) 
04 34400 5.618c (3.756–7.475) 0.202 (0.161–0.243) 0.000350 (0.000301–0.000398) 
04 dialyzed 34400 7.820 (5.16–10.48) 0.190 (0.134–0.246) 0.000492 (0.000463–0.000522) 
05 4750 0.402 (0.312–0.491) 0.240 (0.191–0.289) 0.000758 (0.000659–0.000857) 

n = 6 replicates for each laminarin and each receptor. A minimum three-log concentration range was employed with at least 10 data points for each analysis.

a

BLI/(μg/ml).

b

Confidence intervals are shown in parentheses.

c

Significant change versus Sigma laminarin 01 (129H37842).

d

Significant change versus nondialyzed laminarin.

BMAX, maximum BLI signal.

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