Table III.
Recognition and binding of laminarins by rmDectin-1
LaminarinMolecular Mass (Da)Kd (μg/ml)BMAXNonspecifica
01 4840 0.162 (0.118–0.206)b 0.291 (0.256–0.326) 0.00170 (0.00126–0.00214) 
02 4400 0.713c (0.623–0.802) 0.380 (0.329–0.431) none 
02 dialyzed 4400 0.294d (0.207–0.381) 0.346 (0.305–0.387) none 
03 4800 1.170c (0.724–1.616) 0.286 (0.250–0.322) none 
03 dialyzed 4800 0.453d (0.357–0.548) 0.413d (0.357–0.469) none 
04 34400 2.565 (1.813–3.317) 0.327 (0.306–0.349) none 
04 dialyzed 34400 8.468d (4.741–15.125) 0.366 (0.328–0.403) none 
05 4750 0.257 (0.176–0.337) 0.285 (0.268–0.302) none 
LaminarinMolecular Mass (Da)Kd (μg/ml)BMAXNonspecifica
01 4840 0.162 (0.118–0.206)b 0.291 (0.256–0.326) 0.00170 (0.00126–0.00214) 
02 4400 0.713c (0.623–0.802) 0.380 (0.329–0.431) none 
02 dialyzed 4400 0.294d (0.207–0.381) 0.346 (0.305–0.387) none 
03 4800 1.170c (0.724–1.616) 0.286 (0.250–0.322) none 
03 dialyzed 4800 0.453d (0.357–0.548) 0.413d (0.357–0.469) none 
04 34400 2.565 (1.813–3.317) 0.327 (0.306–0.349) none 
04 dialyzed 34400 8.468d (4.741–15.125) 0.366 (0.328–0.403) none 
05 4750 0.257 (0.176–0.337) 0.285 (0.268–0.302) none 

n = 6 replicates for each laminarin and each receptor. A minimum three-log concentration range was employed with at least 10 data points for each analysis. BMAX, maximum BLI signal.

a

BLI/(μg/ml).

b

Confidence intervals are shown in parentheses.

c

Significant change versus Sigma laminarin 01 (129H37842).

d

Significant change versus nondialyzed laminarin.

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